TY - JOUR AU - Aga, Esayas AU - Bekele, Endashaw AU - Bryngelsson, Tomas PY - 2015/07/06 Y2 - 2024/03/28 TI - Simple Sequence Repeat (SSR) Variation of Ethiopian Forest Coffee (Coffea arabica L.) Populations JF - Journal of Science and Sustainable Development JA - J. sci. sustain. dev. VL - 3 IS - 2 SE - DO - 10.20372/au.jssd.3.2.2015.044 UR - https://aujssd.ethernet.edu.et/index.php/jssd/article/view/44 SP - 1-17 AB - <p style="text-align: justify;"><span style="left: 129.04px; top: 419.033px; font-size: 15px; font-family: sans-serif; transform: scaleX(0.945379);">Genetic diversity and relatedness among 240 individuals of forest coffee trees (</span><span style="left: 653.367px; top: 419.033px; font-size: 15px; font-family: sans-serif; transform: scaleX(1.05383);">Coffea </span><span style="left: 129.04px; top: 437.433px; font-size: 15px; font-family: sans-serif; transform: scaleX(1.02115);">arabica </span><span style="left: 186.433px; top: 437.433px; font-size: 15px; font-family: monospace;">L</span><span style="left: 195.433px; top: 437.433px; font-size: 15px; font-family: sans-serif; transform: scaleX(0.951051);">.) representing 24 populations from Ethiopia were evaluated using 11 simple </span><span style="left: 129.04px; top: 455.433px; font-size: 15px; font-family: sans-serif; transform: scaleX(0.915551);">sequence repeat (SSR) loci. Three of the loci were found to be monomorphic and the other eight </span><span style="left: 129.04px; top: 473.633px; font-size: 15px; font-family: sans-serif; transform: scaleX(0.949123);">loci produced a total of 97 alleles with a mean of 12.1 alleles per locus. Polymorphic </span><span style="left: 129.04px; top: 491.633px; font-size: 15px; font-family: sans-serif; transform: scaleX(0.967997);">information content (PIC) values varied from 0.19 for locus M</span><span style="left: 508.917px; top: 497.85px; font-size: 10px; font-family: sans-serif; transform: scaleX(0.833333);">29</span><span style="left: 518.917px; top: 491.633px; font-size: 15px; font-family: sans-serif; transform: scaleX(0.972642);"> to 0.74 for locus M</span><span style="left: 638.767px; top: 497.85px; font-size: 10px; font-family: sans-serif; transform: scaleX(0.833333);">47</span><span style="left: 648.767px; top: 491.633px; font-size: 15px; font-family: sans-serif; transform: scaleX(1.00241);"> with an </span><span style="left: 129.04px; top: 509.633px; font-size: 15px; font-family: sans-serif; transform: scaleX(0.871094);">average of 0.48 per locus. Jaccard similarity coefficient between all possible pairs of samples </span><span style="left: 129.04px; top: 527.833px; font-size: 15px; font-family: sans-serif; transform: scaleX(0.982881);">varied from 0.41 to 0.96. Total Nei’s genetic diversity (H</span><span style="left: 477.317px; top: 534.05px; font-size: 10px; font-family: sans-serif;">T</span><span style="left: 483.517px; top: 527.833px; font-size: 15px; font-family: sans-serif; transform: scaleX(0.961513);">) = 0.25 while the average diversity </span><span style="left: 129.04px; top: 545.833px; font-size: 15px; font-family: sans-serif; transform: scaleX(1.02056);">within populations (H</span><span style="left: 265.883px; top: 552.05px; font-size: 10px; font-family: sans-serif;">S</span><span style="left: 271.483px; top: 545.833px; font-size: 15px; font-family: sans-serif; transform: scaleX(0.955577);">) = 0.08. The coefficient of genetic differentiation between populations </span><span style="left: 129.04px; top: 564.067px; font-size: 15px; font-family: sans-serif; transform: scaleX(0.989688);">(G</span><span style="left: 144.84px; top: 570.283px; font-size: 10px; font-family: sans-serif; transform: scaleX(0.839286);">ST</span><span style="left: 156.64px; top: 564.067px; font-size: 15px; font-family: sans-serif; transform: scaleX(0.903278);">) = 0.68. Thus, 68% of the total variation was between populations. The partitioning of </span><span style="left: 129.04px; top: 582.067px; font-size: 15px; font-family: sans-serif; transform: scaleX(0.979643);">genetic variation into within and betwee</span><span style="left: 376.083px; top: 582.067px; font-size: 15px; font-family: sans-serif; transform: scaleX(0.957229);">n populations based on Shannon’s information index </span><span style="left: 129.04px; top: 600.267px; font-size: 15px; font-family: sans-serif; transform: scaleX(0.965101);">also revealed more differentiation between populations (0.72) than within populations (0.28), </span><span style="left: 129.04px; top: 618.267px; font-size: 15px; font-family: sans-serif; transform: scaleX(0.971684);">which are in line with the features of predominantly inbreeding populations. The phenogram </span><span style="left: 129.04px; top: 636.267px; font-size: 15px; font-family: sans-serif; transform: scaleX(0.93389);">derived from Jaccard similarity coefficient between populations produced a single tree in which </span><span style="left: 129.04px; top: 654.467px; font-size: 15px; font-family: sans-serif; transform: scaleX(0.953885);">some populations clustered with respect to their region of origin, and a slight differentiation of </span><span style="left: 129.04px; top: 672.467px; font-size: 15px; font-family: sans-serif; transform: scaleX(0.967743);">populations into east and west of the Great Rift Valley was observed except for few </span><span style="left: 129.04px; top: 690.867px; font-size: 15px; font-family: sans-serif; transform: scaleX(0.931552);">populations, which exhibited unique allelic forms. Although, </span><span style="left: 509.517px; top: 690.867px; font-size: 15px; font-family: sans-serif; transform: scaleX(0.968);">C.</span><span style="left: 529.317px; top: 690.867px; font-size: 15px; font-family: sans-serif; transform: scaleX(1.02688);">arabica</span><span style="left: 578.567px; top: 690.867px; font-size: 15px; font-family: sans-serif; transform: scaleX(0.868986);"> is characterized by </span><span style="left: 129.04px; top: 708.867px; font-size: 15px; font-family: sans-serif; transform: scaleX(0.914282);">low genetic diversity owing to its allopolyploidy and autogamous nature, the SSR markers </span><span style="left: 129.04px; top: 727.067px; font-size: 15px; font-family: sans-serif; transform: scaleX(0.888122);">were able to differentiate the forest coffee samples considered in the study. The results are </span><span style="left: 129.04px; top: 745.067px; font-size: 15px; font-family: sans-serif; transform: scaleX(0.92391);">discussed in relation to conservation of forest coffee trees in Ethiopia. </span></p> ER -