In vitro Plantlet Regeneration from Nodal Explants of Anchote [Coccinia abyssinica (Lam.) Cong.] Genotypes
Keywords:Direct regeneration, Genotype, MS media, Nodal explants, Plant growth regulators
In vitro plantlet regeneration experiment using nodal explants in KUWE (G1) and 223098 (G2) genotypes of anchote was conducted by regulating plant growth regulators and nutrient-strength of Murashige and Skoog media. Shoot development was evaluated on media supplemented with different concentrations and combinations of 6-Benzylamimopurine and Kinetin. Root formation was assessed on full and half nutrient-strength media, each with various concentrations of Indol Butyric Acid. Multiple shoots in G1 (4.85) and G2 (5.65) along with 100% induction frequency were obtained at 0.5 mg/L 6-Benzylamimopurine alone and 1mg/L 6-Benzylamimopurine + 0.25 mg/L Kinetin, respectively. Long shoots in both G1 (6.57 cm) and G2 (5.92 cm) was obtained on hormone free medium. Full and half nutrient-strength media without hormone were capable of producing 100% root formation in G1 and G2, respectively. Maximum root numbers in G1 (22.97) and G2 (17.6) were obtained on full nutrient-strength media with 0.25 mg/L and 0.5 mg/L Indol Butyric Acid, respectively. Long roots in G1 (8.02 cm) and G2 (7.07 cm) were recorded from hormone free full nutrient-strength media. Roots developed on hormone free media exhibited normal morphological appearance. After four weeks of acclimatization, plantlets of 76% in G1 and 79% in G2 were survived. In general, plantlet regeneration from nodal explants of both genotypes of anchote has been successfully achieved and the procedures could be applied for in vitro production of propagation materials of the respective genotypes.
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